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Nuclear Magnetic Resonance (NMR) Spectroscopy : NMR can be used to characterize thiols in biological and chemical samples by analyzing their chemical shifts and interactions, providing insights into their molecular environment and reactivity

Serum was centrifuged for 15 min (3000 r/min) and instantly stored at 80 C, which were further used to measure the following parameters: white blood cell count, platelet count, red blood cell count, haemoglobin, total protein, alanine aminotransferase, aspartate aminotransferase, albumin, creatinine, urea, glucose, plasma viscosity, whole blood high shear viscosity, whole blood low shear viscosity, fibrinogen, hematocrit, erythrocyte electrophoretic time, erythrocyte aggregation index, erythrocyte deformation index, the index of rigidity of erythrocyte, total cholesterol, triglyceride, high-density lipoprotein, low-density lipoprotein, Lp-PLA2, endothelin-1, and ICAM-1

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The nitroimidazole prodrug Evofosfamide (TH-302) [493,494,495,496] releases alkylating agents upon reductive cleavage while concomitantly producing cytotoxic free radicals, synergistically exacerbating local oxidative damage