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Description
Briefly, free Lf was removed using centrifugation with an ultracentrifugal filter (100 kDa MWCO, Amicon ) for 1 h at 4 500 g

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Proteins were extracted using RIPA buffer (#ab288006, Abcam)

[DOI] [PubMed] [Google Scholar] 79.Mahoney S.A., Venkatasubramanian R., Darrah M.A., Ludwig K.R., VanDongen N.S., Greenberg N.T., Longtine A.G., Hutton D.A., Brunt V.E., Campisi J., et al
10.1136/bmjopen-2019-031083 BMJ Open
