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2.3 Analysis of organic compounds 2.3.1 Utilization of low-molecular weight organic acids The supernatants obtained as described in section 2.2.1 were centrifuged at 4 C, 11,000 rpm for 10 min and the resulting supernatant was used for the determination of residual contents of salicylic acid, phthalic acid, gentisic acid, protocatechuic acid and catechol by ultraviolet spectrophotometry at 296 nm, 292 nm, 320 nm, 320 nm, and 275 nm, respectively ( 2.3.2 Utilization of BaP The amount of BaP remaining in the culture supernatants obtained from the experiments described in 2.2.2 was determined by GCMS (Aglient 7890/5975c) as described in the literature ( 1 to 140 C

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doi: 10.1038/s41372-021-01010-z 147

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Several strategies have been proposed to suppress MDSC which include: pharmacological targeting, reversing MDSC impact on anti-TB immunity, inhibition of MDSC expansion/recruitment, targeting functions and changing the maturation/differentiation of MDSC into non-suppressive cells (160)