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The rats were deeply anesthetized intraperitoneally using thiopental and diazepam

An alternative approach that has been explored experimentally in the porcine ischemic injury model to limit formation of LA inhibitory fragments while maintaining the ability of LA to induce recovery of barrier function involves addition of small doses of LA over time and suggest that 0.1 M LA applied every 45 min is effective (49)

(2021a)

For medical purposes, some doctors may recommend a weekly B12 shot for up to four weeks