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Molecular biomarkers such as CD44, CD133 and ALDH1 have been extensively validated for their prognostic significance for HNSCC cancer stem cells (CSC) [14, 15]

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Purification The purification procedure is similar as in 47 with the following modifications: 1) The membrane fraction was solubilized by addition of an equal volume of: 10 mM sodium phosphate (NaP i ) at pH 8, 10% glycerol, 0.1 mM EDTA, 1 mM GSH, and 6% Triton X-100 (Sigma) and followed by 45 minutes incubation in 4 C

, Wgrzynowicz M., Ruszkiewicz J., Oja S