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Treatment of the medium with extremes of pH (1.5 or 12) resulted in significant activation of TGF- as shown by radio-receptor assays, while mild acid treatment (pH 4.5) yielded only 20-30% of the activation achieved by pH 1.5

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Scenario 3: Using 5 mL of Bacteriostatic Water This is another option for creating an easy-to-calculate concentration, especially for those who prefer round numbers in their dosing protocols

The basic formula for the concentration is: Total HCG in vial / diluent volume = concentration in IU per ml For example, a 5,000 IU HCG vial mixed with 5 mL of diluent will give: 5,000 IU / 5 mL = 1,000 IU/mL The required volume of injection can then be calculated as: Prescribed dose / concentration = injection volume If the recommended dose is 250 IU: 250 IU / 1,000 IU/mL = 0.25 mL However, the Longeviters' HCG reconstitution calculator takes care of both calculations for you, so you dont have to work through a number of manual conversions yourself

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