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Analyzing the DIA files with the different spectral libraries revealed that the best compromise for maximizing identification of differently charged precursors was to record spectral libraries with NCE of 28 and analyze subsequent DIA runs with NCE of 25 at charge state 2 (Supplementary Fig

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You may need to push the syringe plunger slightly to clear air from the syringe (air displaces the medication, potentially reducing dosage)

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