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Korean J Physiol Pharmacol (2015) 19:43

Xue, C., et al

Cells were collected and resuspended in 10 M DCFH-DA (for ROS), 10 M DAF-FM-DA (for NO), 10 M BODIPY-581/591-C11 (for lipid peroxides), 10 M mito-SOX (for mitochondrial ROS) or 10 M MitoPEDPP (for mitochondrial lipid peroxides) in 300 L phenol red- and serum-free DMEM and then incubated for 20 min at 37 C

Similarly, a study based on swimming exercise for a total of four hours showed an increase in NAD + levels, yet also in this case, no measurements of mitochondrial function were performed 309 , reinforcing the need for more exercise-based investigations to further define how NAD + and mitochondrial homeostasis are perturbed and connected during exercise