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In vivo studies in iron-deficient mice confirm this: erythrocytes exhibit increased PS exposure, reduced glutathione (GSH) levels, and accelerated clearance, with these defects reversed by iron repletion ( 2+ homeostasis: non-selective cation channels in the RBC membranecritical for Ca 2+ entryare upregulated by iron depletion, possibly via ROS-mediated gating

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Additionally, smoking has been found to suppress or downregulate enzymatic antioxidant systems including SOD and catalase in erythrocytes and plasma [55,56], as well as extracellular SOD in serum [57]

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PLP serves as a coenzyme in over one hndred enzymatic reactions, playing a crucial role in amino acid metabolism, neurotransmitter synthesis, gluconeogenesis, and lipid and carbohydrate metabolism