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[DOI] [PMC free article] [PubMed] [Google Scholar] 83.Bruse C, Bergqvist A, Carlstrm K, et al

A P value under 0.05 was considered statistically significant

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(16)Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val- (16) Preparation of Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin (15)Fmoc-Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-1050530105010603DMF3DMF220.53 Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val- In step (15) Fmoc-Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val- Add decapping reagent to the resin, react at 10-50C for 5-30 minutes, drain with a vacuum pump, re-add capping reagent at 10-50C, react for 10-60 minutes, drain, wash with isopropanol for 3 times, DMF for 3 times , washed twice with DMF and twice with methanol, soaked in methanol for 0.5 to 3 hours, drained, and dried with nitrogen to form very dry particles