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Disease onset usually occurs in young adults, more common in females and has a prevalence ranging between 2 and 150 per 100,000.[1] The prevalence of MS in Isfahan-Iran is 43.8 per 100,000.[2] The etiology of MS is complex and multi-factorial involving both genetic and environmental factors, but the main etiology is still unknown.[1] Vitamin B 12 , also known as Cobalamin, plays important structural and functional roles in nervous system and its deficiency leads to demyelination, followed by axonal degeneration and eventually irreversible damage due to axonal death

Furthermore, these studies were rather small-scaled with short duration and design flaws were apparent

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