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For visualization of WGA-HRP, sections were immediately reacted using a modified tetramethyl benzidine protocol (Sigma) (Mesulam, 1982), mounted on gelatin-coated slides, and counterstained with thionin (Matheson Coleman and Bell, Norwood, OH, USA)

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As a 47-year-old guy living in the Denver Metro Area, staying active has always been a big part of who I amwhether thats weight training at the gym, hiking around the Front Range, or skiing in the Rockies during the winter

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