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The expression was induced by adding 1 mM IPTG when OD600 reached 0.7-1.2
For instance, Tsai et al

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In a case study of five patients who were exposed to liraglutide and semaglutide, four patients developed localized pruriginous erythematous infiltrated plaques in reaction to liraglutide injections 1 to 3 months after starting treatment