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INS-1 832/3 cells (a gift from Prof Christopher Newgard, Duke University) (Hohmeier et al ., 2000) were maintained in RPMI at 11 mM glucose, supplemented with 10% FBS, 10 mM HEPES, 1 mM pyruvate, 1 mM pyruvate, 50 M -mercaptoethanol and 1% penicillin/streptomycin

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