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Supporting information ACKNOWLEDGMENTS We thank Shweta Urva, Tamer Coskun, Josh Levine and William Roell (Eli Lilly and Company) for their time and expertise in reviewing sections of this manuscript and Praveen Shetty (Eli Lilly and Company) for assistance with figure creation

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However, their impact on cAMP signaling induced by 10 M GLP-1(9-36) was different: from 85% in wildtype (WT) GLP-1R to 3% (Q234 3.37b A), 10% (V237 3.40b L) and 18% (V237 3.40b F), relative to the maximum response (100%) induced by GLP-1(7-36) ( Fig

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