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In a similar protocol, the in vitro and in vivo effects of metformin on mitochondria function were investigated in vitro in primary murine hepatocytes and Hepa 1-6 cells (murine hepatoma cells derived from the BW7756 tumor) and an in vivo protocol with high-fat diet (HFD) treated mice (138)
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Transfection of HEK293T cells HEK293T cells were seeded on a 3 cm dish 1 day prior to transfection
