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Fissuring or cracking of the vulval skin can occur in severe cases, causing additional discomfort

The optimal parameters were determined to be amide couplings consisting of 2 mL Fmoc protected amino acid (0.5 M in DMF), 2 mL coupling reagent hexafluorophosphate azabenzotriazole tetramethyl uronium (HATU) (0.45 M in DMF), and 0.5 mL N , N -diisopropylethylamine (DIPEA) (1 30 min)

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