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We measured mRNA levels of GLP-1R, PC1/3, PC2 and Pdx1 in cells by PCR, and the trend was also consistent with the trend of their protein expression (Figures 5HK)

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Six-week-old male C57BL/6 mice and adult male Wistar rats (240260 g) were purchased from the Experimental Animal Center of the Shanxi Provincial Peoples Hospital (Taiyuan, China)