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2018) Cell pellets (from 1L insect cell culture) were thawed and suspended in 20mM HEPES pH 7.4, 50mM NaCl, 5mM CaCl 2 , 2mM MgCl 2 supplemented with cOmplete Protease Inhibitor Cocktail tablets (Sigma Aldrich) and benzonase (Merk Millipore)

In order to do an immunostaining the vascular intima, isolectin GS-IB4 Alexa Fluor 594 conjugate (Invitrogen) was applied to the sections

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