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In brief, the PCR product was purified using 0.8x AMPure XP beads (Agencourt, A63881) and quantified with Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Q32856) on an Envision Multilabel Plate Reader (PerkinElmer)

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The nervous system is unique in that it can only use glucose as its energy source

The subjects who, at that time, switched from semaglutide 2.4mg to placebo had regained half of that weight after 48 weeks, whereas those who continued on active treatment achieved an additional weight loss of around 8% in the same time period

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