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The monolayer was scratched with a sterile 200 L pipette tip, and cells were then treated with the TGF-1 (10 ng/ml) + IL-1 (10 ng/ml) for 24 h at 37 C
For retatrutide specifically, one preclinical rat study (Windram et al., 2025) has shown it blunts subjective alcohol effects but no human data exists yet
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