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The culture supernatant was harvested by centrifugation at 6000 g for 20 min and clarified by filtration

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This release is rapid, and is known to peak at around 10 min from the initial glucose challenge, whilst the second phase of insulin release, which is sustained, consists in the release of granules from the larger Reserve pool (RP), containing ~9599% of insulin granules, and lasts until glucose stimulation ends (3060 min under normal physiologic conditions) (35)

If you tolerated semaglutide without significant urinary changes, you will likely have a similar experience on tirzepatide