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The membranes were washed by homogenization in 30mM Hepes, pH 7.5, 150mM NaCl, 100uM TCEP, 2.5 mg/ml Leupeptin, 0.16 mg/ml Benzamidine then collected by centrifugation at 37,800g for 25 minutes

The same sequence every week builds muscle memory and reduces errors

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It never has been
