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doi:10.1111/j.1468-1293.2007.00488.x 209

This observation also highlights the complexity that modifying a residue within a cholesterol binding site might have on the overall pattern of receptor interactions with cholesterol and other plasma membrane lipids, as increased segregation to cholesterol-rich lipid rafts occurs despite an overall reduction in total cholesterol binding under vehicle conditions observed for the V229A mutant receptor

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These are among the reasons ongoing medical supervision matters throughout treatment not just at the start