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Cells were harvested by centrifugation at 4,000 g and resuspended in lysis buffer (20 mM Tris, 300 mM NaCl, 5 mM imidazole, 0.7 % CHAPS, pH 8.0) supplemented with protease inhibitors (Thermo Fisher Scientific) and bovine pancreatic DNase I (Sigma-Aldrich)
[36] discussed incretin-based therapies as a novel treatment for depression in T2DM
On another note, the body favours the synthesis of cortisol over other hormones like progesterone and estrogen
van Strien T
doi: 10.1016/j.pneurobio.2010.07.003
