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The oral formulation's market penetration is constrained by lower bioavailability relative to injection therapy, requiring higher doses to achieve equivalent glycemic control, which increases manufacturing costs and limits payer reimbursement enthusiasm in cost-conscious healthcare systems

The flow-through was collected the next day and concentrated with a Amicon Ultra centrifugal filter (molecular weight cut-off of 100 kDa, Millipore) and then subjected to a Superdex 200 Increase 10/300GL column (Cytiva) with running buffer (pH 7.5) containing 20 mM HEPES, 100 mM NaCl, 10 mM MgCl 2 , 100 M TCEP, 10 M ligand, 0.00075% (w/v) LMNG, 0.00025% (w/v) GDN and 0.0002% (w/v) CHS