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On the day of the assay, plates were removed from the incubator and each well was washed twice with 100 l HBSS (Gibco, Life Technologies) and pre-incubated for 30 min at 37 C with 85 l HBSS per well

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While this delay doesnt usually change the total amount of a medication absorbed, it can impact the timing of absorption for certain drugs

Further studies are needed to fully understand all the pathways involved, but current evidence strongly suggests cardiovascular and hepatic benefits for semaglutide based on its potent anti-inflammatory effects

Hjerpsted JB, Flint A, Brooks A, Axelsen MB, Kvist T, Blundell J