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Strict dieting is not required
In one study, people who slowly tapered off the meds over the course of nine weeks were able to continue losing weight during the tapering period
2023;22(4): doi:10.36849/JDD.6550 Citation: Ouellette S, Frias G, Shah R, et al
doi: 10.3390/nu11112566 148 YangFRuanYCYangYJWangKLiangSSHanYBet al
For protein extraction, each tissue was placed into a tube containing a lysis buffer consisting of 20 mM Tris HCl with pH 7.6, 0.5 M Sucrose, 0.15 M KCl, 20 mM DTT supplemented with 100 L/mL of a protease inhibitor cocktail (Sigma-Aldrich, MO, USA) in a 1:3 (v/v) ratio
