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Nat Med 19 : 567575

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In controlled trials, patients receive close monitoring and support

Finally, after checking the effects in controlled studies, it would be reasonable to expand to studies in patients, with more heterogeneous samples, but with experimental N significantly higher than that of the laboratory experiments, as well as those used in epidemiological studies, to again avoid incorrect inferences (Porsani et al., 2020)

For example, LC-based approaches can leverage a battery of orthogonal separation mechanisms (e.g., RPLC, HILIC, SEC, IEX), hyphenated to different detection systems (e.g., UV, ELSD, RI, MALS, MS) to expand the coverage of measurable properties