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RNA extraction and quantitative polymerase chain reaction: Total cellular RNA was isolated using the RNeasy Plus Kit (QIAGEN, Germantown, MD, USA), and 500 ng of RNA was subjected to reverse transcription using SuperScript III Reverse Transcriptase and an oligo (dT) primer (Thermo Fisher Scientific, Inc.) to yield cDNA, followed by amplification with Power SYBR Green (Thermo Fisher Scientific, Inc.) using the Applied Biosystems Model 7900HT in the Genomics Division of the Iowa Institute of Human Genetics (IIHG)

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To Report Adverse Reactions, Contact the FDA: Phone: 1-800-FDA-1088 Online: www.fda.gov/medwatch Disclaimer These statements are intended for informational purposes and do not replace professional medical advice

Gasbjerg LS, Helsted MM, Hartmann B, et al

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