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ELISA The supernatants of each group of BV2 cells were harvested, and the levels of IL-1 (cat

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As MASLD progresses, metabolic disturbances such as excessive triglyceride storage, de novo lipogenesis, lipotoxicity, ER stress, mitochondrial dysfunction, and oxidative stress culminate in hepatocyte injury, leading to apoptosis, necrosis, and necroptosis and the release of damage-associated molecular patterns (DAMPs) and proinflammatory mediators that drive immune cell infiltration and liver inflammation [138]