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Bilateral injections of AAV vectors encoding for stimulatory (AAV8-hSyn-DIO-hM3Dq-mCherry, Addgene, 44361-AAV8) or inhibitory (AAV8-hSyn-dF-HA-KORD-IRES-mCitrine, Addgene, 65417-AAV8) Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) were then performed in the OB (A/P: +4.8 mm from bregma, M/L: 1 mm and D/V: 1.7 mm from brain surface) at a rate of 0.125 L/min for 4 min, on Glp1r -Cre RD and WD mice

Plug in the defaults to see what each dose delivers: Skincare (2.5 mL water with GHK-Cu anchor) Payload: GHK-Cu 2 mg BPC-157 0.4 mg TB-4 0.4 mg KPV 0.4 mg

MarchettiPLupiRBuglianiMKirkpatrickCLSebastianiGGriecoFAet al

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