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Importantly, repeated daily intrathecal injections of morroniside over seven days did not result in the development of tolerance to its anti-allodynic effects [73]

doi: 10.1038/npp.2009.75 18 BremnerJ

Non-specific bindings were blocked by incubation with 5% non-fat dry milk in Tris-buffered saline Tween (TBST) for 2 h

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Although the findings in the CANVAS trial showed a higher risk of amputation when taking canafliglozin compared to placebo [92], no significant difference in the risk of this complication was observed in the CREDENCE study [88]