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For RT-qPCRs, total RNA was extracted from liver and muscle using Nucleospin RNA kit (Macherey-Nagel, 740955.250), then reverse transcription was performed on 1 g of RNA using SensiFAST cDNA synthesis Kit (Meridian Biosciences, BIO-65053)

Specialists in obesity medicine play a crucial role in creating treatment strategies that help patients lose weight while maintaining lean muscle mass

Because the effective dose reaching the bloodstream is likely lower than with injections, the gastrointestinal side effects that often accompany injectable tirzepatide, particularly constipation , diarrhea , and nausea, tend to be milder with the oral formulation

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Algunos pacientes eligen la administracin matutina por razones prcticas de adherencia, pero no hay evidencia de que este horario ofrezca ventajas de eficacia sobre la administracin nocturna