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Quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) Total RNA was isolated with Superbrilliant 6 min High-quality RNA Extraction Kit (zsgentech, Tianjin, China), according to the manufacturer's instructions

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This makes it safer for longer-term use and eliminates concerns about developing insulin resistance

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The administration of MNs is minimally invasive, painless, and safe, making MNs an ideal option for obesity treatment

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