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Peptide purification was performed using an Agilent 1260 Infinity high-performance liquid chromatography (HPLC) system equipped with a Phenomenex Aeris C18 column (250 mm 4.6 mm, 3.6 m particle size, and 100 pore size)

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However, alongside these cardiovascular benefits, clinical trials and real-world observations have consistently noted a modest increase in resting heart rate among patients taking GLP-1 receptor agonists