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However, a healthy and steady intake of calories is important to maintain energy levels, especially for those running long distances or training intensely

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Gene expression analysis via qRT-PCR Total RNA was isolated from 50 to 100 mg of liver tissue using the RNeasy Mini kit (Qiagen, Tokyo, Japan), followed by reverse transcription using a high-capacity RNA-to-cDNA kit (Thermo Fisher Scientific, Tokyo, Japan) according to the manufacturers instructions