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Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI) (Beyotime, China) at RT for 10 min

The mice were placed on a rotating rod (Ugo Basile) that maintained a constant speed of 24 rpm, and each trial lasted for 60 s for motor learning test
Animal studies exposed to GLP-1 receptor agonists during pregnancy have shown adverse outcomes such as decreased fetal growth and abnormalities, but human studies have not shown a significant risk of developing birth defects [187,188,189,190]
