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GLP-1 Fc-fusion was eluted with a gradient (15 column volumes) 50 mM sodium acetate, pH 3.65

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If there are any particles left, continue to swirl gently

In general, most hydrophobic BAs are considered as FXR agonists, while hydrophilic non-12-OH TMCA, UDCA, and T(G)UDCA serve as endogenous FXR antagonists 4,9,10
