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In brief, brains were postfixed in 4% paraformaldehyde (Fisher #AC169650010) in 0.1 m phosphate buffer at +4C for 24 h then equilibrated in 30% sucrose (Fisher S5-3) in 0.1 m PBS (Fisher #BP399-20) at +4C before slicing on a freezing microtome (Leica) in a 1:12 series of 40-m-thick coronal sections

Updated on April 30th, 2026 Poor oral hygiene causes 85-90% of halitosis cases by allowing bacteria to accumulate on teeth, gums, and especially the tongue's back surface where anaerobic bacteria produce sulfur compounds

L.HyvarinenN.LillyN.KayK.DossatA.PariseE.et al (2011)

As the novelty of the medications wears off, side effects are becoming a focal point as users complain of stomach discomfort, muscle loss and now loose skin