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Scores 15 indicate severe depressive symptoms and should prompt direct referral to mental health services

PLOS Glob Public Health

doi: 10.1210/en.2011-1443

After pre-conditioning in the presence or absence of 50 nM Exendin-4 for additional 18 hours, culture medium was changed to serum-free DMEM containing 1 mM sodium pyruvate, without glucose and sodium bicarbonate
