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DOI.org (Crossref) , Greenfield, Jerry R., et al

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Importantly, localized increases in GLP-1, such as that occur in the gut and portal vein, often have a more profound effect than systemic elevations, emphasizing the therapeutic potential of targeting regional activity rather than relying solely on systemic concentrations

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Once the rat was placed inside, the concentration was reduced to 3% until the respiratory rate stabilized, then maintained at 2% throughout the surgical procedure