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Fat biopsies from washed in PBS, minced with a scalpel and disaggregated with digestion medium containing low glucose DMEM, 10 mg/ml BSA, 1.5 mg/ml type IV collagenase and 0.6% antibiotic mixture for 4050 min at 37 C and agitation

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For nonadherence, the 1year absolute risks of nonadherence stratified according to baseline HbA1c are shown in Figure S1B

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