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454,455 Unlike liver or tumor tissues, the dense extracellular matrix and dynamic contraction of the heart create significant physical barriers, limiting the distribution of RNA, protein, and cell therapies

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The study had some limitations

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Cells were resuspended in ice-cold PBS plus 0.1% BSA and processed using a BD LSR II flow cytometer (10,000 cells/sample) for receptor trafficking, or BD LSRFortessa X-20 for surface downregulation

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