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Similarly, the other incretin hormone, GIP is efficiently cleaved by DPP-4 in vivo (40), and DPP-4 inhibition increases intact GIP levels and enhances its effects (41)

(1997) Distribution of glucagon-like peptide-1 and other preproglucagon-derived peptides in the rat hypothalamus and brainstem

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Add too little and you risk not fully dissolving the peptide, leaving some stuck to the vial walls