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Immunohistochemical detection of alpha and beta cells was performed using rabbit anti-insulin (3014, 1:800, Cell Signaling Technology) and mouse anti glucagon (G2654, 1:1,000, Merck) as primary antibodies and goat anti-rabbit AF750 (A21039, 1:100, Invitrogen) and donkey anti-mouse AF555 (A32773, 1:200, Invitrogen) as secondary antibodies

No funding was received for this study or to assist with the preparation of this manuscript

Letting it sit at room temperature for a while can make the injection feel smoother

Chronic Kidney Disease Prognosis Consortium and Chronic Kidney Disease Epidemiology Collaboration

~7-day half-life enabling weekly dosing