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The membranes were blocked for 1 h at room temperature with 5% nonfat milk in TBST (20 mM TrisHCl, 0.5 M NaCl, 0.1% Tween 20)

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This gradual approach minimizes gastrointestinal side effects and allows the body to adjust to the medication

Role of a Dual Glucose-Dependent Insulinotropic Peptide (GIP)/Glucagon-like Peptide-1 Receptor Agonist (Twincretin) in Glycemic Control: From Pathophysiology to Treatment