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Growth media was replaced with stimulation buffer [phenol-free DMEM containing 0.1% (w/v) bovine serum albumin (BSA) and 1 mM 3-isobutyl-1-methylxanthine] and incubated for 1 h at 37 C in 5% CO 2

Ohno, T., Aune, D

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BAT activity correlates inversely with age, body mass index, and fasting glucose levels