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Protein concentrations of all samples were quantitatively and uniformly extracted with BCA

I'm less and less of a stickler about, you know, even the number of sets, for example, or reps, if you're getting the similar effort is

Antonie Van Leeuwenhoek

My suspicion is that most people regain the weight because the treatment was short-term, focused on weight loss rather than developing better habits, and resulted in loss of muscle, which reduces your basal metabolic rate and makes you more likely to gain weight