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Ligands were added immediately prior to reading on a Mithras LB 940 multimode microplate reader (emission filters at 400 nm and 515 nm)
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But the total weekly volume should be moderate

[DOI] [PMC free article] [PubMed] [Google Scholar] 45.Chen Z, Kelly JR, Morales JE, Sun RC, De A, Burkin DJ, et al
